Indexat a

Llicència i ús

Citacions

Altmetrics

Grant support

This research was partially funded by the BBVA Foundation as part of the project 'Investigation on the potential role of pets as animal reservoirs for SARS-CoV-2'. The authors also acknowledge the crowdfunding initiative #Yomecorono, available online at https://www.yomecorono.com (accessed on 12 December 2024). IRTA (2021 SGR 00825 and 2021 SGR 01385) and IrsiCaixa (2021 SGR 00452) are supported by CERCA Programme/Generalitat de Catalunya.

Anàlisi d'autories institucional

Compartir

Comparison of Three Commercial ELISA Kits for Detection of Antibodies Against SARS-CoV-2 in Serum Samples from Different Animal Species

Publicat a:Viruses-Basel. 17 (5): 716- - 2025-05-16 17(5), DOI: 10.3390/v17050716

Autors: Fernandez-Bastit, Leira; Marfil, Silvia; Pradenas, Edwards; Blanco, Julia; Vergara-Alert, Julia; Segales, Joaquim

Afiliacions

Resum

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) caused the coronavirus disease 19 (COVID-19) pandemic, significantly impacting global health, economies, and social stability. In February 2020, the first cases of SARS-CoV-2 infections in animals were documented, highlighting the potential risks posed by regular human-animal interactions in facilitating viral transmission. In consequence, it is essential to validate surveillance methods for SARS-CoV-2 in animals. In the present study, 101 sera from different animal species (36 cats, 41 dogs, 4 ferrets, 10 wild boar, 6 domestic goats, and 4 lions) were tested using three different ELISA kits to evaluate humoral responses against SARS-CoV-2. ELISA results were compared and correlated with a pseudovirus neutralization test (pVNT), considered as the reference assay. ELISA-1, targeting the receptor binding domain (RBD) neutralizing antibodies (nAbs) of SARS-CoV-2, exhibited the highest diagnostic performance, and proved to be a reliable tool for initial screenings in high-throughput animal studies. In contrast, ELISA-2 (also targeting RBD nAbs) and ELISA-3 (targeting nucleoprotein antibodies) demonstrated lower sensitivity for detecting seropositive animals.

Paraules clau