{rfName}
Si

Indexed in

License and use

Icono OpenAccess

Citations

Altmetrics

Grant support

This research was funded by the Spanish Ministry of Science and Innovation grant PID2021-125599OB-100. LC was supported by the Juan de la Cierva Program (2022), the Spanish Ministry of Science and Innovation. AMA was supported by the ICA grant 001-2022. IRTA is supported by the CERCA program/Generalitat de Catalunya.

Analysis of institutional authors

Coronado, LianiAuthorGanges, LlilianneCorresponding Author

Share

Publications
>
Article

Simultaneous Detection of Classical and African Swine Fever Viruses by Duplex Taqman Real-Time PCR Assay in Pigs Infected with Both Diseases

Publicated to:Pathogens. 14 (5): 473- - 2025-05-13 14(5), DOI: 10.3390/pathogens14050473

Authors: Coronado, Liani; Muñoz-Aguilera, Adriana; Wang, Miaomiao; Muñoz, Ivan; Riquelme, Cristina; Heredia, Saray; Stepniewska, Katarzyna; Gallardo, Carmina; Ganges, Llilianne

Affiliations

CSIC, Ctr Invest San Anim CISA, European Union Reference Lab African Swine Fever E, Inst Nacl Invest & Tecnol Agr & Alimentaria INIA, Madrid 28130, Spain - Author
Ctr Recerca Sanitat Anim CReSA, Unitat Mixta Invest IRTA UAB Sanitat Anim, Bellaterra 08193, Barcelona, Spain - Author
Inst Colombiano Agropecuario ICA, Bogota 110911, Colombia - Author
IRTA CReSA, WOAH Reference Lab Class Swine Fever, Barcelona 08193, Spain - Author
IRTA, Ctr Recerca Sanitat Anim CReSA, Programa Sanitat Anim, Bellaterra 08193, Barcelona, Spain - Author
Natl Vet Res Inst, Dept Swine Dis, Partyzantow 57 Ave, PL-24100 Pulawy, Poland - Author
See more

Abstract

The increasing spread of African swine fever (ASF) in recent years and the presence of classical swine fever (CSF) subclinical forms in endemic countries suggests that the possibility of coinfection with ASF virus (ASFV) and CSF virus (CSFV) in pigs cannot be ruled out in areas where both diseases are prevalent. Thus, rapid and reliable diagnosis through molecular testing is essential for the timely implementation of control measures to prevent the spread of these devastating swine diseases. Here, we have coupled two of the most validated PCR assays for the detection of CSFV and ASFV in a single reaction tube. The combination of the two tests for the detection of two target nucleic acids did not affect the analytical sensitivity, and the duplex RT-qPCR assay was comparable with the standard molecular techniques. The detection limits for CSFV RNA and ASFV DNA were 0.12 TCID50/reaction and 0.25 TCID50/reaction, respectively. The test showed high repeatability and reproducibility, the coefficient of variation was below 2%, and excellent performance was demonstrated in clinical samples. The duplex assay shows great potential to become a robust diagnostic tool for the rapid and reliable detection and differentiation of CSFV and ASFV in areas where both viruses may be circulating.

Keywords

African swine feverAfrican swine fever virusAnimalsAsfvClassical swine feverClassical swine fever virusCoinfectionCsfvDifferential detectionDoubly infected pigsDuplex qpcrEarly diagnosisReal-time polymerase chain reactionReproducibility of resultsRna, viralSensitivity and specificitySurveillancSurveillanceSwine

Quality index

Bibliometric impact. Analysis of the contribution and dissemination channel

The work has been published in the journal Pathogens due to its progression and the good impact it has achieved in recent years, according to the agency WoS (JCR), it has become a reference in its field. In the year of publication of the work, 2025, it was in position 70/161, thus managing to position itself as a Q2 (Segundo Cuartil), in the category Microbiology. Notably, the journal is positioned en el Cuartil Q2 para la agencia Scopus (SJR) en la categoría .

Impact and social visibility

From the perspective of influence or social adoption, and based on metrics associated with mentions and interactions provided by agencies specializing in calculating the so-called "Alternative or Social Metrics," we can highlight as of 2025-06-11:

With a more dissemination-oriented intent and targeting more general audiences, we can observe other more global scores such as:

    It is essential to present evidence supporting full alignment with institutional principles and guidelines on Open Science and the Conservation and Dissemination of Intellectual Heritage. A clear example of this is:

    • The work has been submitted to a journal whose editorial policy allows open Open Access publication.

    Leadership analysis of institutional authors

    This work has been carried out with international collaboration, specifically with researchers from: Colombia; Poland.

    There is a significant leadership presence as some of the institution’s authors appear as the first or last signer, detailed as follows: First Author (CORONADO BAEZ, LIANI) and Last Author (GANGES ESPINOSA, LLILIANNE).

    the author responsible for correspondence tasks has been GANGES ESPINOSA, LLILIANNE.